I would like to do quantitative estimation of amino acids in blood sample for inborn errors of metabolism. I have a new agilent HPLC 1260 model. Can anyone provide the protocol? Thanks in advance.
I don't know if you are looking for targeted or un-targeted analysis of amino acids, but this publications might be a good start: MIller IV et al.: A quantitative method for acylcarnitines and amino acids using high resolution chromatography and tandem mass spectrometry in newborn screening dried blood spot analysis, Journal of Chromatgraphy B (2012), 903:142-149.
There is a relatively "new" type of HPLC column you could try that combined reversed phase with ion pairing properties and works quite well with AAs; for example: Primesep A available from SIELC Technologies is a reverse-phase analytical column with embedded strong acidic ion-pairing groups. However, bear in mind of you are analysing CFS, plasma or urine of paediatric patients suspected to suffer from inborn errors of AA metabolism your focus should be on elevated levels of key AA metabolites (such as SCFAs or keto-acids) and not just decreased AA levels (as e.g. in OTC deficiency).