And how can I create a test report for growth promotion test?
Thank you very much for support.
Take appropriate volume of the broth in a side arm flask. Inoculate the media with the culture. Check its optimal density just after inoculating the culture. Incubate it for sometime, say 24 hours and then check the optical density. Expecting an increase in the optical density if it is a growth promoting media. the side arm of the flask can be inserted into the space where cuvettes are put into the colorimeter without opening the falsk. Alternatively, you can take a small fraction out in aseptic conditions and check for the absorbance. You can plot a graph of O.D versus time of incubation and see the promotion of growth in various media and compare.
Hope this helps! :)
To add to what has been highlighted above, you have to note the type of bacteria you are working with: Aerobic vs Anaerobic so that you can device a suitable media for it. This means that you would have to note the energy or carbon source and electron acceptor chemicals if working with anaerobes while in the case of aerobes, oxygen is the electron acceptor, which the bacteria obtains directly from the atmosphere as far as the flasks containing bacterial innoculum are under constant agitation. Growth of the bacteria can be monitored using various media and the best medium which shows the best growth of the bacteria is then selected for subsequent experiments. This is obtained from a growth curve of increase in OD vs time.
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