Using qiagen blood and tissue kit.
Is there any good method for this?
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I'm making quantum well with 2 different bandgap materials, but I'm not sure quantum confinement effect was occurred because the well width is ~ 60 nm. How can I make sure that effect? Is there...
02 July 2024 8,426 1 View
My research object is changing terminal functional group of PDMS from -OH to -PO(OH)2. I know just one experiment recipe using furan, ethylamine, POCl3, D.I water. But it not work well. So i want...
09 June 2024 5,290 0 View
I have created an axis using Datum points with a specific vector in Abaqus, intending to rotate a cylinder around this axis. However, in the Assembly module under Rotate Instance, after selecting...
09 May 2024 9,298 1 View
Is the PARIS AGREEMENT A REAL DEAL FOR CLIMATE CHANGE? This was the big question raised in my postgraduate open forum on the New GREEN DEAL. There were no clear-cut answers to that HOLY GRAIL...
07 May 2024 3,536 1 View
During the transcription process, we encountered a situation where only PS was transferred I'm so curious about the solution. We make a PS solution using 300 mg of Polystyrene and 3 ml of...
06 May 2024 6,786 0 View
Even though multi-photon absorption is fascinating phenomenon, recently I am agonizing about its applicability. Second harmonic generation is used in lasing materials or so, however, I have never...
28 April 2024 2,524 1 View
The Legal Impact of AI development on business and rural society in the Asia-Pacific and ASEAN region is at a crossroads. How will AI impact the local community (urban and rural) now?
22 April 2024 5,682 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
