How can I dissolve guanosine to use it in cell culture?

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Maria Grazia Tozzi

It is very difficult to obtain even a 10-20 mM solution of guanosine, you may tray an alkaline pH and exposition at high temperature, good luck

Tomáš Hluska

what about DMSO? We use that for derivatives of adenine and it works well.

Ferran Riaño-Canalias

It worked in DMSO, both 10 and 100 mM. Thank you so much.

Maria Zizzo

you may use a solution 10%NaOH to prepare mother solution and adjust the pH with HCl To avoid precipitation, you need to use the solution once prepared (within 30min).