How to work a GLM with the Principal Components from a PCA?

I've got a big amount of environmental data as independent variables, so I used a PCA to work better with them. But I have problems in extracting/converting the data from de Principal Components...

12 January 2020 4,257 7 View

How can I get the area and perimeter of a group of pixels of a raster layer?

For my Final Degree Project I'm working with several Buffers of a reclassified landcover map (raster format) and I would like to have the total area and perimeter of a certain class (forest) to...

11 December 2019 2,036 3 View

Any source for real time control in PYNQ boards?

Real time control in parallel robots is a requisite. Python has popularity for scientific computation thanks to Scipy and Numpy libraries. Hardware implementation of control routines reduce...

21 July 2019 3,783 1 View

Has anyone ever experienced a reduction in NF-kB nuclear localization when using poly-D-lysine coated coverslips?

In the past we grew cells on uncoated coverslips to monitor NF-kB activation via immunofluorescence. Recently we started to use this method again, but switched to poly-D-lysine coated coverslips...

15 November 2017 7,870 2 View

How can I dissolve guanosine to use it in cell culture?

I want to prepare a 100 mM guanosine (G6264 from Sigma) solution to add it in a HCT116 cell culture at a 100 uM concentration. It didn't work neither in water nor in 1N acetic acid...

01 February 2014 1,841 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View