I'm trying to differentiate sh-sy5y cells with retinoic acid, but them do not differentiate, they are dying. Is there a specific way to dilute the RA? any special condition?
What is the culture medium you use for differentiation? Usually DMEM/F12 medium with 1%FBS (low FBS content) + 10uM RA is used as differentiation medium for SH-SY5Y. You need to change the medium every 48 hours.
Also, the cell density is an important factor. You need to optimize on the cell density. Too many cells will crowd the area with less space left for the outgrowing neurites and having too little cells will result in cell death.