Can anybody suggest me which is the best method to determine MIC in mycobacterium bovis BCG, or any related publication to it. Is it possible to determine the MIC using 96 well plate? Thank you
Hi,
You can do it in a 96-well plate. Most methods I have come across determine MIC using spread plate methods and calculating CFU/ml after treating the bacteria in several dilutions of the antibiotic/drug. However other methods measure fluorescence after the addition of resazurin. I have attached a paper for you if it helps! Good luck!
Hi Madhu, thank you for your generous explainaition. just wondering, when you said you add 200ul of 0.02 OD600 bovis bcg, do you know that at that od what is predicted cfu/ml of bcg? is it 10^5 cfu/ml?
this is my first time working with mycobacteria so many technical details still unknown. thanks
Nor, When I start the MIC experiment (with OD 0.02), I also plate culture on 7H11 agar plates. Although I take MIC reading on day 5, but we get the CFU count only about 3 to 4 weeks later (due to slow replication rate of mycobacteria). Usually the CFU is about 10^5- 10^6 /ml.
Maybe it's been a long time, but I recommend working with the graphpad prism software. In this regard, it analyzes very well.
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