Hi All,
In my project, I need to design at least 7 orthogonal DNA sequences that cannot bind with each other or have harmful secondary structures.
The sequences also should contain primer binding sites that are orthogonal to each other.
Can anybody recommand me some softwares or websites that could do this. Thanks very much.
Best,
Gang
When you say that the sequences cannot bind to each other, do you mean that they cannot be complementary to each other? Also, could you clarify what you mean by "harmful secondary structures"? If 7 orthogonal sequences will suffice, then it should be fairly easy to write some code yourself to do the job.
Hi Khan,
Thanks. When I say the sequences cannot bind to each other, I mean they don't have too many base pairs complementary, say less than 4. Harmful secondary structures means no hairpin, palindromic sequence et al. As for the code, can you give me some hints on that? Thanks.
Gang
- Badges
- Science method