07 July 2016 6 7K Report

Hi All,

My project needs some streptavidin-oligo and antibody-oligo conjugates. But usually I could only use 20-60 ug antibody to do the conjugation reaction as the primary antibodies are usually very expensive. Then how can I purify the conjugates after the reaction from the unreacted oligo and antibody? I  think size exclusion chromatography is hard to get a signal on that amount? Could you guys please give me some suggestions on that? Thanks a lot.

Best,

Gang

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