I need to deoxygenate myoglobin samples with cost-effective chemical compounds and read its absorption spectrum.
I am a new researcher in Public Health just joined this group and set networking with you all
17 December 2023 9,657 1 View
Hello, I would like help in detecting and marking in western blotting. We in our laboratory do not have a chemiluminescence detection system. Would it be possible to detect chemiluminescence using...
02 June 2022 1,953 0 View
I use SMARTvector Inducible Lentiral shRNA vector, which include 2 promoters: costitutive promoter, which control the expression of puromycin resistence and TET-On 3G transactivator and inducible...
15 June 2021 3,120 1 View
I'm developing an impedimetric biosensor for lipase.
28 May 2020 4,290 1 View
I need to get data of bird's distribution at marine archipelagoes, but the data need have been islands specific.
05 September 2019 2,860 0 View
I am using xenografts ( lung cancer tissue) and need to evaluate fibrosis. I am planning to do Picrousirius red staining, but I am not sure about the precise quantification. I was wondering if...
21 May 2018 346 3 View
The virus is said to contain genes from avian, swine, and human influenza A. How could a hybrid like this have happened? What events could have occurred to allow the reassortment of such genes?
17 April 2017 293 3 View
I would like to simulate a piezoelectric harvester (figure 1) and perform a transient analysis in a bimorph piezoelectric beam. I know how to define piezoelectric material, but I'm having some...
18 January 2017 4,146 1 View
I need to quantify and identify oxidative damage on DNA (and proteins). I had some boiling extracts of gram-negative bacteria that showed interesting variation on UV-absorption after an oxidative...
26 September 2015 9,463 4 View
Mouse; Lung
06 May 2015 9,213 8 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View