How can I determine DNA oxidative damage using uv-spectrophotometry?

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How we explore a good research finding in this group?

I am a new researcher in Public Health just joined this group and set networking with you all

17 December 2023 9,657 1 View

Kodak Gel Logic 200 in Western Blotting detection?

Hello, I would like help in detecting and marking in western blotting. We in our laboratory do not have a chemiluminescence detection system. Would it be possible to detect chemiluminescence using...

02 June 2022 1,953 0 View

Someone has experience with shRNA inducible system?

I use SMARTvector Inducible Lentiral shRNA vector, which include 2 promoters: costitutive promoter, which control the expression of puromycin resistence and TET-On 3G transactivator and inducible...

15 June 2021 3,120 1 View

How can I calculate the detection limit for an impedimetric biosensor?

I'm developing an impedimetric biosensor for lipase.

28 May 2020 4,290 1 View

Anyone know where I can get bird's distributional data of oceanic islands?

I need to get data of bird's distribution at marine archipelagoes, but the data need have been islands specific.

05 September 2019 2,860 0 View

Which is the best approach for a qualitative and quantitative fibrosis characterization in a tumor tissue?

I am using xenografts ( lung cancer tissue) and need to evaluate fibrosis. I am planning to do Picrousirius red staining, but I am not sure about the precise quantification. I was wondering if...

21 May 2018 346 3 View

What is the nature of the relationship between the three “parents” of the H1N1 virus?

The virus is said to contain genes from avian, swine, and human influenza A. How could a hybrid like this have happened? What events could have occurred to allow the reassortment of such genes?

17 April 2017 293 3 View

How do I define a harmonic base excitation boundary condition in APDL?

I would like to simulate a piezoelectric harvester (figure 1) and perform a transient analysis in a bimorph piezoelectric beam. I know how to define piezoelectric material, but I'm having some...

18 January 2017 4,146 1 View

In your opinion what is the most accurate angiogenesis marker?

Mouse; Lung

06 May 2015 9,213 8 View

Can we simply determine B-H curve of a certain material as we obtained the M-H hysteresis curve from VSM test?

07 February 2015 443 8 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View