How can i deal with flourescense inhomogeneity in MST protein-protein interaction?

24 November 2023 0 1K Report

I have tried several of these protein-protein interaction with different concentrations, but i don't get that average capillary scan. It always like the image uploaded.

Please does anyone have a way out for these?

Badges
Science topic

More Faith P Samson's questions See All

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why does community engagement play an important role in addressing challenges related to blood donation and transfusion services in Western Kenya?

This question is based on research done by Kavulavu et al. et al. in 2022, entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya." In this...

10 April 2024 9,791 0 View

What measures could future researchers or scientists employ to standardize HDV diagnosis with PCR in the future?

According to the research of Pan et al. (2023) entitled, “Diagnostic Efficacy of serological Antibody detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis,” what...

07 April 2024 3,234 2 View

Implementation methods matrix according to hbr?

HBR handbook project management

20 March 2024 8,210 1 View

what is the process of olfactory sensation to perception?

also the related words in that topic

10 March 2024 7,348 0 View

Is there a structural explanation to why a same his-tag proteins may have different affinity to MST red-tris dye?

Both proteins are in PET28a(+) but one has high affinity (like 10 times higher) than the other his-tag protein. I am confused as to why it is so. Please does some have same experience?

16 November 2023 2,167 0 View

Afrocentric Intervention Paradigm: An Overview of Successful Application by a Grassroots Organization ?

I am looking for this journal below Afrocentric Intervention Paradigm: An Overview of Successful Application by a Grassroots Organization. Journal of Human Behavior in the Social Environment

16 November 2023 4,296 0 View

Which articles discuss foreign language policy implementation?

Please provide me with current, up to date peer-reviewed articles that discuss the image and reputation of the school when it offers foreign languages as a subject.

15 November 2023 7,509 0 View

Please could you check - how to calculate a SE of the SMD?

I need to calculate SE for an SMD. Could anyone confirm for me, with a worked example? I have from here revman5-calculator.pdf (cochrane.org) that SE for SMD = Sqrt (N/N1N2 + SMDsqrd /...

12 September 2023 968 0 View

How can I edit my publication dat in my publication ?

I uploaded my research articles as I publish but publication date was by mistakenly added wrong Now How to correct that please guide me

10 June 2023 9,186 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,988 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,198 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,006 0 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View