According to the research of Pan et al. (2023) entitled, “Diagnostic Efficacy of serological Antibody detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis,” what measures could future researchers or scientists employ to standardize HDV diagnosis with PCR in the future?
Good day Ms. Bautista,
Aside from the mentioned serological methods employed in the study namely Radioactive Immunoassay, Enzyme Immunoassay, ELISA and Automatic Immunoassay analysis, the use of a one-step RT-qPCR method for the quantification of HDV-RNA was studied and developed in Brazil. This method is essential to reduce the risk of false positives and inconclusive results.
Reference: Queiroz, J. A., Roca, T. P., Souza, R. B., Passos-Silva, A. M., Alves de Souza, L. F., Da Sila, A. L., Borzacov, L. M., Cassia (2023, July 26). Development of quantitative multiplex RT-qpcr one step assay for detection of hepatitis delta virus. Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus. https://doi.org/10.1038/s41598-023-37756-z
Good day Ms. Miranda,
According to Turgeon (2021), the presentation of HDV antigen in serum are the two main ways to detect hepatitis D infection. EIA test procedures are used for HDV IgM antibody testing, and qualitative enzyme-linked immunosorbent assay (ELISA) is used to identify HDV antigen. The production of IgM or IgG HDV antibodies, occur at the same time as hepatitis A or B antibodies. However, examining the HDV antibodies using IgG and IgM present in an individual do not require special considerations.
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