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05 September 2016 1,180 3 View

Do I need isolate CD4 positive cells by MACS first for IL-22 intracellular staining in mouse MLN lymphocytes?

Recently I'm working on detecting IL22-producing CD4 positive cells in mouse MLN by flow cytometry. However, after using PMA/ionomycin plus GolgiPlug neither 4 hrs or 6 hrs culture, IL-22 positive...

01 September 2015 778 3 View

Does anyone know where anti-mouse alpha1beta1 integrin for immuno (frozen sections) can be purchased?

I was wondering if anyone knows where the above can be puchased? Or is the only option to use an antibody that targets rat instead of mouse tissue? Any help would be very much appreciated.

30 April 2014 7,194 4 View

Struggling to determine what statistical test to use for my MSc thesis?

Let me begin by stating that statistics are unfortunately no strength of mine. I have conducted a questionnaire assessing loss of smell/taste in healthcare workers during the COVID-19 pandemic...

26 August 2020 5,335 4 View

Nursing article about corona virus?

Hello Dear I need to catch scholarly articles about people attitude about contagious diseases like corona virus and how to mange their fear despite of maintaining their sensation about it

23 March 2020 6,936 12 View

If humans are so "complex", is it always harder to understand human behavior [patterns] than to understand similarly functioning patterns in animals?

If humans are so "complex", is it always harder to understand human behavior [patterns] than to understand similarly functioning patterns in other animals? NO !! Of course not: we see as other...

04 May 2019 672 1 View

What are the different ways to improve mindfulness?

Mindfulness: a mental state achieved by focusing one's awareness on the present moment, while calmly acknowledging and accepting one's feelings, thoughts, and bodily sensations, used as a...

03 April 2019 2,181 13 View

Most appropriate statistical analysis tool for category data?

I am currently designing a questionnaire to explore the location in the body that participants experience sensations during episodes of particular emotions. The questionnaire will ask...

31 December 2018 6,566 9 View

Can anyone confirm success of this therapy?

11 December 2018 770 0 View

Is there a questionnaire for proprioceptive sensations?

I am writing a review on somatosensory sensations arising during different therapeutic interventions and noticed that exteroception and interoception both have several questionnaires with...

11 December 2018 762 1 View

Likert Scale and Others?

Dear partners I would like to share a reflection I am a statistician, and many times I find some queries strange and I apologize for not understanding quickly. But it seems strange to me to...

07 August 2018 5,465 0 View

A Beginning of a Human Ethogram: seeing the inception of cognitive-developmental stages as involving a couple of phases of non-conscious perception?

WHAT? "Non-conscious perception": isn't that an oxymoron; isn't anything like that SENSATION?? NO. Such conclusions involve one believing he/she knows all the sorts of species-typical basic,...

02 March 2018 2,798 2 View

Doesn't our Understanding of the Memory Capacities/Capabilities Inform Us that Innate Guidance to Learning During Ontogeny would INCREASE LEARNING?

Yes. And, I could also give you the answer [as I indicated (or implied), elsewhere] that innate guidance for new types of learning just gives us new types of learning, and we still have all the...

08 September 2017 9,185 2 View