I'm trying to model the human DAT1 Variable Number of Tandem Repeats (VNTR) 9- and 10-repeat alleles in mice (this is purely theoretical and for a project - will not be carried out in practice). This variant is localized in the human 3'-UTR, but does not exist in mice (the gene does). Different alleles of this variant have been associated with different levels of DAT1 expression, but results are not conclusive. I have already proposed in vitro work to examine transcriptional activity (Luc assay) and mRNA stability (pulse-chase), but I would like to create an in vivo model.
Can I simply replace the mouse 3'-UTR with the human code? What in vivo considerations do I need to include? Is there another way to mimic this variant e.g. site-directed mutagenesis? siRNA?
Thank you
Yes but results probably will not fully supported human data. Can you use a full human BAC with several Kb to include human promoter and full DNA seq with the variant there?
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