How can i count foci of gamma h2ax with software?

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Alokkumar Jha

CHARM Hough transform based algorithm is a great match can share you my python script if required.

Regards,

Alok

Pegah Johansson

There are many (expensive) softwares that can detect nuclei and count particles in it. But if you plan to do this once and move on, you can use Image J which is free. There is a fair bit of information on how to use Image J for foci counting on the internet and some have written macros for this purpose you can use!

We use the ScanR software which comes with a high throughput microscope.

Good luck

Pegah