Hello Ronita De

229.29g ---- 1 L ---- 1M

229.29 mg ------ 1L ---- 1mM

Make a 1mM stock solution of Lamivudine by dissolving 2.292 mg of Lamivudine in10ml DMSO.

Use C1 x V1 = C2 X V2

Where C1= Concentration of Lamivudine = 2292ug

V1= Xml

C2= Required concentration (100 ug)

V2= Required volume (1ml)

2292ug x X = 100ug x 1ml

Therefore X= 0.04363ml

Add 43.63ul of stock (1mM) to 956.37ul of culture media to give 100ug of Lamivudine per ml culture media.

Best.

https://www.google.com/url?sa=t&source=web&rct=j&url=https://pubmed.ncbi.nlm.nih.gov/18364269/&ved=2ahUKEwjS4bDzxOT5AhUoQPEDHfl7CdcQFnoECAYQAQ&usg=AOvVaw3MllaHqilBER9rXufIY0mi