Hello Ronita De

277.279g ---- 1 L ---- 1M

277.279 mg ------ 1L ---- 1mM

Make a 1mM stock solution of Entecavir by dissolving 2.7727 mg of Entecavir in 10ml DMSO.

Dilute the stock (1:1000) in culture media to get 1uM diluted stock of Entecavir.

Use C1 x V1 = C2 X V2

Where C1= Concentration of Entecavir = 2772.7ng

V1= Xml

C2= Required concentration (30ng)

V2= Required volume (1000ml)

2772.7ng x X = 30ng x 1000ml

Therefore X= 10.81ml

Add 10.81ml of diluted stock (1uM) to 989.19ml of culture media to give 30ng of Entecavir in 1 litre culture media.

Best.

Thank you Malcolm Nobre