I have designed a repair template targeting the stop codon of my GOI such that it inserts a HA tag and an antibiotic resistance gene at the C-terminal end. I can see that the cells survive post treatment with the antibiotic (control cells are dead) and also see a band corresponding for the HA tag when blotted for a western. However, I want to confirm if this is indeed what i am aiming for i.e. If the tag has been inserted at the right position and orientation. Can anyone suggest ways in which i can do the same?