Radhika Nair

4 Questions 4 Answers 0 Followers

Questions related from Radhika Nair

How do i study invitro interaction of two proteins that have been shown to interact in vivo?

I am working on two proteins of the UPS, that have been shown to interact with each other in vivo. The ultimate goal is to study the complex using X-ray crystallography. I would also like to study...

23 October 2018 6,469 5 View

How can I confirm CRISPR cloning based tag insertion?

I have designed a repair template targeting the stop codon of my GOI such that it inserts a HA tag and an antibiotic resistance gene at the C-terminal end. I can see that the cells survive post...

14 February 2018 1,621 1 View

Has anyone used primers >100bp for cloning? Please help?

I am trying to stitch in a 38 amino acid tag to the N-terminal end of my protein (3200bp) to be cloned into a lentiviral vector (~7000bp). The forward primer for the same, along with the overhang...

26 October 2017 4,467 6 View

Is it advisable to add a C-terminal linker between a streptavidin binding peptide (SBP) tag and the protein of interest?

I am looking to clone a 120kDa protein with a C-terminal SBP tag (38 aa) into a lentiviral vector. Is it ok to add a linker between the gene and the SBP tag (followed by the stop codon)? If so,...

25 July 2017 6,202 3 View