Radhika Nair

2 Questions 4 Answers 0 Followers

Questions related from Radhika Nair

Has anyone used primers >100bp for cloning? Please help?

I am trying to stitch in a 38 amino acid tag to the N-terminal end of my protein (3200bp) to be cloned into a lentiviral vector (~7000bp). The forward primer for the same, along with the overhang...

26 October 2017 4,415 6 View

Is it advisable to add a C-terminal linker between a streptavidin binding peptide (SBP) tag and the protein of interest?

I am looking to clone a 120kDa protein with a C-terminal SBP tag (38 aa) into a lentiviral vector. Is it ok to add a linker between the gene and the SBP tag (followed by the stop codon)? If so,...

25 July 2017 6,184 3 View