For example after running a column chromatography I realized that five of the collected fractions contained high enzyme activity (after running an assay on each). So I've pooled the five fractions together but the total volume is too high. So, now, how can I reduce the volume of the pooled fraction (as in concentrating the pooled fraction)?
The instruments that might be useful and found in my University are: Freeze drier, steam bath/water bath, and rotary evaporator. Are there any chemical means of concentrating the pooled fraction?
Why not use a vivaspin column? https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314774443672/litdoc28935653AA_20110831115051.pdf
you could use appropriate MW cutoff Concentrators column to do your job in no time. Example- http://www.sartorius.us/us/products/laboratory/ultrafiltration-devices/centrifugal-ultrafiltration-devices/
You could use anything. Concentrate with correct cutoff and use the spin filter to remove any precipitate you got because of concentration. This is what we do every do in crystallography lab. I have not used the instruments you have mentioned for this purpose so I cant comment.
Thanks for your valuable suggestions, I will suggest these products to my supervisor and let her decide.
Many companies sell these products so you could even ask for samples and they will send it to you. If you try 2-3 companies you should get some positive response.
good luck
I have had some success using reverse dialysis (using high mol wt dextran solutions). You can reduce volumes by as much as 90%.
Millipore MW centrifugal filters is a trademark used in most of the labs in our Uni.
I'd recommend nanosep (Pall Life sciences) I used them when I was working with downstream processing of monoclonal antibodies, and they were efficient.
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