Allan Wee Ren Ng

5 Questions 25 Answers 0 Followers

Questions related from Allan Wee Ren Ng

Is anyone working with Fabry cell lines?

I am currently looking for laboratories or cell line repositories that are working or have worked with Fabry disease who are willing to provide me some transformed Fabry cell lines preferably...

04 August 2017 1,975 2 View

What are the other types of buffering salts used?

I'm going to extract polyphenol oxidases (PPOs) from a fruit peel and would like to find out their pH optimum so I need to incubate the purified enzymes into different test tubes, containing...

18 May 2013 2,326 2 View

How can I concentrate a pooled fraction that contains my desired enzymes?

For example after running a column chromatography I realized that five of the collected fractions contained high enzyme activity (after running an assay on each). So I've pooled the five fractions...

08 May 2013 7,806 7 View

Any protocols in enzyme purification that retain high enzyme activity?

I'm going to work on polyphenol oxidase (PPO) extracted from a fruit peel, and from what I read recently I came out with a series of purification steps involving ammonium sulphate fractionation,...

08 May 2013 1,409 21 View

Which antifoam reagent should I use to reduce toxicity to the cells?

I know about silicone and alcohol waxes.

03 February 2013 2,768 1 View