S D Yogesha

3 Questions 26 Answers 0 Followers

Questions related from S D Yogesha

Has anyone had experience on stability of enveloped virus (HSV1) during purification?

During my experiment, I couldn't account for all the virus in most of the purification processes, so I thought it might be getting inactivated during the process. Yield is measured by plaque...

29 March 2018 1,493 4 View

Native gel with extra Mn2+ couldnt not polymerize enough to give good wells. Why?

I was making two native gels with 5mM Mg2+ in one and Mn2+ in other. Mg2+ was fine. Mn2+ gel did not give any wells and all collapsed and remaining gel solution turned different color. Please see...

11 February 2016 3,909 1 View

If a phosphatase is inhibited by a tungstate ion, what could be the possible mechanism? What are the amino acids it targets?

Example: BeF3 is a potent inhibitor of phosphatases that form phosphoaspartate intermediates.

18 March 2014 8,959 0 View