So i should calculate plasmid copy number and i want to try two methods:
the first:=(amount (ng)*6.022x10^23)/(length(bp)*1x10^9*650)
the second one: depends on the molecular weight of each nucleotide
Can you please tell me which one is more accurate?
i think it might be the second because it doesn't take the assumption that the molecular weight of a base pair is 650 daltons but take into consideration the weight of each nucleotide.
Thank you in advance
Yes it is the second. And when you take the mass each nucleotide, you need to think that your nucleotide where engage in a covalent linkage and "loose" some atom. In addition, plasmid DNA is a double strand molecule.
There is a a free download booklet from Roche which helps you with this and similar calculations. Just search for "LabFAQs and Roche.
I updated the above attached file - now just using "Daltons" for unified atomic mass units
Idt's take: https://eu.idtdna.com/pages/education/decoded/article/calculations-converting-from-nanograms-to-copy-number
Better 2nd option, having into account sequence and DNA structural configuration.
You can use calculate MW using this link: https://www.bioinformatics.org/sms2/dna_mw.html
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