I have small angle x- ray scattering data (q and I values) for my samples. I tried Guinier fitting method but am not convinced with the results obtained, since, it is confusing which section to chose for obtaining the slope in the Guinier plot.
The Guinier plot is performed at small q, so it is not related to the thickness of bilayers. The thickness distance of liposome sample is represented by Bragg reflections (for MLV liposomes) or by a bump (ULV) at q around 1/nm, roughly.
Thank you Mercedes Cocera for your answer. Could you please provide me with a little more detail about representing by Bragg reflections (for MLV) ? I would like to get your attention to the point that what I am trying to find is not the repeat distance obtainable from the first reflection (using 2pi/q1) but the thickness of the lamellar bilayer excluding the water layer. (I hope you won't mind if I am asking too basic things, since I am very new in this particular area)
I recommend you Pabst et al. papers. They work with lipid bilayers and SAXS, and the papers are very clear.
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