Ananda Kafle

9 Questions 16 Answers 0 Followers

Questions related from Ananda Kafle

How to do molecular dynamic simulation?

I want to carryout molecular dynamic simulation of some amphiphilic molecules to study their assembling behavior. My particular interest is in the lamellar bilayer arrangements. Someone, please...

17 May 2019 10,214 7 View

Is normalizing twice, the SAXS data okay ?

I use Anton Paar SAXCess camera to obtain small angle x- ray scattering data for my phosphatidylcholine (+sterol) samples. The profiles are normalized against the beam profile while integrating...

21 October 2016 5,263 2 View

How to distinguish between lamellar gel, liquid ordered, liquid crystalline L- alpha and ripple phase by using polarized optical microscopy?

There are a number of well defined POM textures for lamellar phases, e.g. mosaic, coarse mosaic, oily streaks and Maltese crosses. As per some references, it seems that for gel phase, the mosaic...

17 March 2016 9,017 3 View

Which of the following two ways of baseline subtraction is correct?

Following figure contains two ways of interpolation and subtraction of baseline in a DSC endotherm of a mixture of phosphatidylcholines. Compared to one another, the curve obtained by second...

15 February 2016 5,794 7 View

Are PFG-NMR and magic angle spinning NMR available in ordinary NMR instrument or are they different setups?

Are pulsed field gradient, PFG-NMR and magic angle spinning, MAS-NMR facilities available in ordinary instruments or are different instrumental setups? I mean is it possible to achieve these two...

14 December 2015 2,559 3 View

Does the liquid ordered Lo phase of a phosphatidylcholine thermally change into liquid disordered L- alpha phase?

In my experiments with aqueous suspensions of soy phosphatidylcholine, two DSC transition peaks were observed. First is thought to be the pretransition form gel to ripple phase and the second, the...

15 October 2015 318 0 View

How can I see water in a phosphatidylcholine sample under a polarizing optical microscope in cross Nicole conditions?

I frequently find authors claiming the presence of water in a sample by indicating some black spots in POM textures. But such black spots can be possible in many cases of isotropic phases and...

26 May 2015 1,217 2 View

Can anybody explain the best way of Guinier fitting for SAXS data?

First I tried it manually for the SAXS data obtained for my phosphatidylcholine samples. Although, the Rg (Gyration radius) values obtained are somewhat satisfactory, I am not much convinced with...

22 May 2015 9,335 3 View

How can I calculate bilayer thickness (thickness of the lipid bilayer excluding water) for hydrated soy lecithin?

I have small angle x- ray scattering data (q and I values) for my samples. I tried Guinier fitting method but am not convinced with the results obtained, since, it is confusing which section to...

14 May 2015 5,118 5 View