I want to buy chromatography columns for the affinity-based separation of proteins. The size (diameter) of the column needs to be very small (less than 100um) in order to match the design. Are there any companies to manufacture such products? Do they also coat the columns with required/requested ligands?
100um? You can try to make a microfluidic chip with a channel depth and width of 100um and length as your desired column length. On your chip, you can make two micropillar structures with opening (pore) width of 10um as supporting screens. Load your resins before bonding the chip and you will get what you want.
https://www.thermofisher.com/ng/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-affinity-purification.html
check this out
Dear Mohammad
you can check the phynexus web site
https://phynexus.com/products/
they produce a lot of custum coloumns for affinity purification in robotic platform but as Peter told you, i tihnk that 100um is really to small size.
good luck
Manuele
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