Mohammad Mahdi Keshavarz

13 Questions 6 Answers 0 Followers

Questions related from Mohammad Mahdi Keshavarz

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

07 January 2021 5,918 3 View

What are the differences between an ultrasonic homogenizer and a vortex mixer? What are their ads and cons?

I want to suspense and mix my micro/nano-particles inside a solution. What are the differences between an ultrasonic homogenizer and a vortex mixture?

06 October 2020 5,758 3 View

Does the difference in pH of two separate flows in an aqueous two phase system (ATPS) affect the other flow and its contents?

I have an ATPS microfluidic system and I want to see if the two input flows in the Y channel affect each other's pH. I believe since it's a microfluidic system and the flows have a low Reynolds...

19 September 2020 6,935 3 View

How much time does it take to measure the IgG content in human blood utilizing current laboratory methods?

What are the current methods to quantify, measure, and detect the content of Immunoglobulin G proteins in human blood? How much time does it take for such tests to complete? How much does it take...

25 October 2019 2,198 3 View

Is it possible to elute IgGs bound to Protein G ligands using temperature and heating (without denaturing IgGs)?

I have modified an affinity chromatography column coated with Protein G to efficiently separate IgGs. Is it possible to elute the bound IgGs by adjusting temperature instead of utilizing elution...

21 October 2019 8,782 2 View

What are the best softwares to simulate Microfluidic Affinity Chromatography process?

I want to simulate my microfluidic design which utilizes affinity chromatography at micro-scale. I have already worked with software such as Comsol Multiphysics. Is there any other software? Which...

15 October 2019 2,369 1 View

How can I buy/order custom micro sized columns for affinity chromatography?

I want to buy chromatography columns for the affinity-based separation of proteins. The size (diameter) of the column needs to be very small (less than 100um) in order to match the design. Are...

06 October 2019 4,974 4 View

Is Microfluidic mass separation/production of plasma proteins economically efficient versus the more traditional methods?

Plasma proteins are currently separated and stored utilizing traditional ethanol fractionation methods. Is it possible to economically and commercially substitute this process with novel methods...

18 August 2019 3,798 1 View

Is it better to produce human blood immunoglobulin proteins using separation from plasma or using the genetically engineered/recombinant methods?

There are different ways to produce IgG. One is using intact IgG from blood/plasma donors. The other one is using genetically engineered/recombinant methods which use yeast cell as host. Which one...

16 April 2019 3,415 12 View

Are there any methods to detect and find the chemical formula of a polymer (carbon, hydrogen, oxygen)?

I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...

08 March 2019 679 7 View

What forces/stresses occur inside the GI Tract- Small Intestine of a human body?

I am doing some research about the biomechanics of GI Tract and I need force/stress analysis of the small intestine. I have found multiple essays on the subject but few of them helped me.

31 May 2018 5,289 2 View

Is there any intermittent crank-slider mechanism to stop the mechanism in the middle and separate the continuous motion into two equal intervals?

I want the slider to stay in its peak position for some seconds and then keep on moving. It could be a 1 revolution rotation.

21 April 2018 2,803 4 View

Particle Size And Shape In Particle Tracing Module

Hi. I faced a problem in a simulation with particle tracing module. Is there any way to change the shape and geometry of the particles in the particle tracing modules? For example turn the sphere...

01 January 1970 7,594 3 View