Hi everyone
I have 3 questions:
1. What parameters are important in encapsulating an electroactive molecule in liposomes?
2. What techniques I should be use for verification of liposomes structure and encapsulation efficacy?
3. What is the best and easy way for separating un-encapsulated molecules from liposomes with sizes in micrometer range?
The un-encapsulated molecules is sensitive to PH, Oxygen and high temperature.
Thank you for your valuable response
1. You should analyze the un-encapsulated molecules You need to operate HLB number 0- most hydrofobic 20-most hydrofilic.
2. DLS (dynamic light scattering)
3. If you use the extruder get liposomes of defined dimensions rather homogeneous. However, if you use a sonification method you will get a different diameter. You can take in the washer with ultrasound (150W)
Thanks #Marcin Staśko.
I did not have access to advanced tools for preparing liposomes. But finally I cooperated with some of my friends and we fabricated a liposome extruder for preparing of nanoliposomes with desired structures.
1- I used dialysis bag for washing of liposomes.
2- I think the best approach is cryo-TEM but we can also use DLS to evaluate hydrodynamic diameters of liposomes.
3- I used ultrasonic approach but this approach should be optimized. Moreover, the batch to batch repeatably is not so good.
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Hi Dr,
In reply to your question about : "separation of unloaded electroactive species from micro-size liposomes", have a look at below manuscript / patent and attached papers. Let me know if you have further questions / comments:
Mozafari, M. R. (2005). Method and apparatus for producing carrier complexes. UK Patent No. GB 0404993.8, Int. Appl. No. PCT/GB05/000825 (03/03/2005), 14.
Khorasani, S., Danaei, M., & Mozafari, M. R. (2018). Nanoliposome technology for the food and nutraceutical industries. Trends in food science & technology, 79, 106-115.
Dear Dr Tohid Mahmoudi
To answer your other question:
"What parameters are important in encapsulating an electroactive molecule in liposomes?"
The following parameters must be considered:
A. characteristics of the active compound to be encapsulated/entrapped
(e.g. molecular weight, melting point, solubility, pKa);
B. the intended route of administration;
C. lipid/phospholipid composition and physicochemical characteristics
of these ingredients of the nanovesicles;
D. physicochemical properties of the medium and solvents/co-solvents
in which the drug, phospholipids and the resultant nanoliposomes
will be suspended;
E. potential toxicity and effective concentration of substances which
are intended to be encapsulated or entrapped in the nanocarriers;
F. desired shelf-life, encapsulation efficiency, drug release profile,
optimum size, polydispersity index (PDI) and zeta potential of the
formulation;
G. scale of production, batch size and cost-effectiveness of the manufacturing
method.
Source:
Mohammadabadi, M. R., & Mozafari, M. R. (2018). Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form. Journal of Drug Delivery Science and Technology, 47, 445-453.
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