i have done bacteriostatic process and find results but i can not analyse the result
Bacteriostatic effects of hyaluronic acid.
Result:- Recombinant hyaluronic acid. exerted varied bacteriostatic effects on all the bacterial strains tested depending on its molecular weight (MW) and concentration. The high concentrations of the medium MW HA had the greatest bacteriostatic effect, particularly on the Actinobacillus actinomycetemcomitans, Prevotella oris, Staphylococcus aureus, and Propionibacterium acnes strains. The 1.0 mg/ml concentration of high MW HA had the greatest overall bacteriostatic effect, inhibiting the growth of all 6 bacterial strains tested. Among the bacterial strains studied, HA was found to have no bactericidal effects, regardless of concentration or molecular weight.
If you are using liquid media monitor turbidity and if there is no increase in turbidity then the compound is bacetriostatic, now transfer a loopful culture to fresh medium without the inhibitory compound and check for increase in turbidity, due to decrease in concentration of the compound microbial growth should occur leading to increased turbidity
I would suggest using filtration, centrifugation, or a neutralizing agent to remove the bacteriostatic inhibitor. Centrifugation and re-washing cells may be best (or easiest). Do plate counts before and after adding the inhibitor, and after centrifuging/washing the cells....direct plate counts. I would be cautious with the idea of taking a carryover of sample aliquot into fresh media (without inhibitor) because you don't know if all the cells are growing in the fresh media (without inhibitor) in the absence of the inhibitor; what you may be seeing is simply a small fraction that escaped bactericidal activity growing and appearing as a bacteriostatic result. So to design the experiment:
1. Plate count culture before adding bacteriostatic inhibitor.
2. Plate count culture after adding bacteriostatic inhibitor.
3. Centrifuge/resuspend in fresh media to wash away bacteriostatic inhibitor, plate count again.
If #3 is really low, then it's bactericidal; if #3 is close to #1, then the bacteriostatic inhibitor was removed and giving you the normal plate count again.
Counting of colony forming unit before and after addition of inhibitor is the best way to analyze the mode of action either bacteriostatic or bactericidal. The answer given by Peter M Muriana is most appropriate.
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