Why do we use klenow fragment for sequencing?

I came across klenow fragment when I was reading about HiTS-FLIP method. I know klenow fragment doesn't posses 5'->3" exonuclease activity but what advantage does it provide for ?

01 November 2020 7,598 3 View

How can i calculate Cp ( Coefficient of power) in Vertical axis turbine?

I have a question on Cp in the wind turbine. I have calculated the moment in the z-axis as my rotor also rotors along the z-axis. I got the moment values from my simulation. I applied the...

23 July 2020 5,622 4 View

How to craete Atmospheric Boundary layer in ANSYS Fluent 16 ?

30 March 2016 6,070 3 View

How to justify this coefficient of pressure (Cp) in this Building ?

22 March 2016 4,677 6 View

How to relate the strouhal number FFT fluent to manual calculation St= FD/V?

08 March 2016 5,189 4 View

Can some one help me find strouhal number in this graph?

I had my simulation of ellipse with the dimension of Major axis=5m amd minor axis= 0.2m and i get contours and all the things, I don't know how to find strouhal number in this graph. please help...

23 August 2015 7,545 3 View

What is mean by the mesh size of tellurium powder and does the reactivity is different according to the mesh size?

20 October 2014 9,115 10 View

How many excitons will form when we irradiate the semiconducting nanomaterials with a xenon lamp?

I am getting dual emission for a hetero nano structure and I need an explanation. Please comment on this particular aspect.

14 October 2014 4,719 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View