Hi
Stain the cells with CD45, CD3 and CD56. Apply CD45 gate to your CD3 population and then apply this double gate to your CD56 population. Use bright fluorochrome conjugated antibody for CD56.
Additionally you may include CD16 and CD8 as they will provide additional information about sub NK populations.
Take a look at the following article in Blood
Azzi T1, Lünemann A1, Murer A2, Ueda S3, Béziat V4, Malmberg KJ5, Staubli G6, Gysin C7, Berger C1, Münz C2, Chijioke O8, Nadal D1. Role for early-differentiated natural killer cells in infectious mononucleosis. Blood. 2014 Oct 16;124(16):2533-43.
Abstract
A growing body of evidence suggests that the human natural killer (NK)-cell compartment is phenotypically and functionally heterogeneous and is composed of several differentiation stages. Moreover, NK-cell subsets have been shown to exhibit adaptive immune features during herpes virus infection in experimental mice and to expand preferentially during viral infections in humans. However, both phenotype and role of NK cells during acute symptomatic Epstein-Barr virus (EBV) infection, termed infectious mononucleosis (IM), remain unclear. Here, we longitudinally assessed the kinetics, the differentiation, and the proliferation of subsets of NK cells in pediatric IM patients. Our results indicate that acute IM is characterized by the preferential proliferation of early-differentiated CD56(dim) NKG2A(+) immunoglobulin-like receptor(-) NK cells. Moreover, this NK-cell subset exhibits features of terminal differentiation and persists at higher frequency during at least the first 6 months after acute IM. Finally, we demonstrate that this NK-cell subset preferentially degranulates and proliferates on exposure to EBV-infected B cells expressing lytic antigens. Thus, early-differentiated NK cells might play a key role in the immune control of primary infection with this persistent tumor-associated virus.
© 2014 by The American Society of Hematology.
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