How can I adjust 4 multiply by 10 power 6 per ml conidia using a haemocytometer?

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Laith Al-Ani

167

Hi Aqleem

You can read this "Reference1"

https://cals.arizona.edu/classes/plp427L/lab2.html

Also "Reference2"

http://crcooper01.people.ysu.edu/Using%20a%20Counting%20Chamber%20090709.pdf

Also "Reference3"

https://libcatalog.cimmyt.org/download/cim/93682.pdf

Also "Reference4"

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779437/

Also "Reference5"

https://pub.epsilon.slu.se/id/file/6638

And "Reference6"

https://link.springer.com/chapter/10.1007/978-981-10-0021-8_12/fulltext.html

Ajonok Hano

Useful information Laith Al-Ani thanks

Leo Atwood

Several good references here on ResearchGate.

Check this discussion on a 5x10(4) spore concentration:

Note the answer from: Susann Auer · - · Technische Universität Dresden:

"Since you obviously have to adjust your spores to an exact concentration I recommend that you count as many squares as possible and repeat your counting with another sample of your spore suspension. So you will assess proper mixing of the sample prior to counting.:

How to find the find the concentration of fungal spores? - ResearchGate. https://www.researchgate.net/post/How_to_find_the_find_the_concentration_of_fungal_spores2 [accessed Feb 7, 2017].

Also:

https://en.wikipedia.org/wiki/Hemocytometer#Usage

https://www.researchgate.net/post/How_to_prepare_a_fungal_spore_suspension_from_a_plate_or_tube_for_later_inoculation_into_liquid_media

https://www.researchgate.net/post/How_to_find_the_find_the_concentration_of_fungal_spores2

https://www.researchgate.net/post/How_to_perform_cell_counting_bacteria_using_hemocytometer

www.uky.edu/Ag/Wheat/wheat_breeding/protocols/Using%20Hemacytometer.doc