Hello,
I have the crystal structure of a construct to which I would like to add N- and C-terminal residues. Secondary structure prediction programs indicate that these amino acids are forming alpha helices, so I would like to add this helices to the already solved structure of my construct, instead of using homology modeling to generate a completely new structure. Is it possible? How can I do it?
Hi Ignacio,
You can use rosetta tool to do it. You can specify the secondary structure of your residue in it.
Try builder tool in pymol (http://pymol.org/edu/?q=educational/ ) and/or you can also use coot (http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ ) to add your residues.
I agree with Navneet. Pymol can do it... but it will be slow. Coot is probably your best choice.
You could also use Discovery studio Visualizer which is a freeware and You can decide wheather you want to add alpha helix, beta sheet residues, etc.
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