Marcela Cárdenas-Tueme

5 Questions 10 Answers 0 Followers

Questions related from Marcela Cárdenas-Tueme

How can I achieve large-scale expansion of human mesenchymal stem cells?

Hello! Im working with primary human MSC, I obtained them from adipose tissue, however, I've been trying to expand them unsuccessfully, I use normal T-75 tissue culture flask, DMEM with 15% SFB,...

23 June 2023 6,128 2 View

My iPSCs morphology is changing and I don't know why?

Hello everyone I'm working with ATCC-BYS0111 Human [Hispanic Male] Induced Pluripotent Stem (IPS) Cells ACS-1025™ and the thing is that their morphology is changing a lot, I've been following the...

08 November 2022 3,609 4 View

¿Has anyone ever used the iBox Explorer in an ex vivo experiment?

Hello everyone I've got an IBox machine that I'd like to use, however, I was wondering if any of you has used it ex vivo? I mean, I've got a breast cancer mouse model where I'm studying metastasis...

18 October 2022 4,326 0 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

15 October 2020 4,719 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

26 August 2020 6,631 4 View