Marcela Cárdenas-Tueme

9 Questions 13 Answers 0 Followers

Questions related from Marcela Cárdenas-Tueme

How do you pick your iPSCs colonies?

Hi, I'm trying to pick my iPSCs colonies but I've never done this before, how do you do it? Do you have a microscope inside the hood?

11 October 2023 2,827 1 View

How can I achieve large-scale expansion of human mesenchymal stem cells?

Hello! Im working with primary human MSC, I obtained them from adipose tissue, however, I've been trying to expand them unsuccessfully, I use normal T-75 tissue culture flask, DMEM with 15% SFB,...

23 June 2023 6,188 2 View

My iPSCs morphology is changing and I don't know why?

Hello everyone I'm working with ATCC-BYS0111 Human [Hispanic Male] Induced Pluripotent Stem (IPS) Cells ACS-1025™ and the thing is that their morphology is changing a lot, I've been following the...

08 November 2022 3,676 4 View

¿Has anyone ever used the iBox Explorer in an ex vivo experiment?

Hello everyone I've got an IBox machine that I'd like to use, however, I was wondering if any of you has used it ex vivo? I mean, I've got a breast cancer mouse model where I'm studying metastasis...

18 October 2022 4,398 0 View

Myelin purification for phagocytosis assay?

Hello everyone, I was wondering if anyone knows another protocol for myelin purification other than the one published by Norton & Poduslo, 1973. I want to do a phagocytosis assay ex vivo with...

19 November 2020 6,445 2 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

15 October 2020 4,835 3 View

Why is MTT precipitating at the bottom.of my 96-well plate?

Hi everyone I've been having problems with my MTT assay, for some reason MTT isn't dissolving correctly and it seems like it doesn't enter the cells, it just forms crystals at the bottom of the...

29 August 2020 7,366 5 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

26 August 2020 6,738 4 View

Which brand of dissecting tools to use on Drosophila's brain do you recommend the most?

Hi all! I'm about to start working on Drosophila's brain and as it turns out, the lab doesn't have the right forceps nor other tools to dissect the brain, which brand and size would you recommend...

31 August 2016 6,537 4 View