I have incubated my fungi for 10 days, then scraped and sieved it using a strainer. I prepared dilutions of 1:10, 1:20, and 1:50, and measured absorbance at 650 nm using a spectrophotometer. I obtained several readings, calculated the average, but in order to inoculate I will need a concentration of around 1 × 10^5. I have read about approaches such as using a standard curve or Beer–Lambert law, which require measurements with known concentrations. This is confusing me, and I feel completely lost. Initially, I tried using a hemocytometer, but my fungi (Macrophomina phaseolina) did not appear clearly under the microscope. Could you kindly advise?
Phil Geis, Thanks for the suggestion. I will look into it. I am not sure, as this fungus grows like a lawn, wool all over the plate, of which I doubt that doing CFU quantification will be possible.
The technique has been around for a long time - folks use it for poorly sporulating fungi - some to separate effect on sopre germination from mycelial growth.Article Evaluation of a Modified EUCAST Fragmented-Mycelium Inoculum...
Don't know Macrophomina phaseolina but its an ascomycete so should be amenable.
Phil Geis, thank you for your response and suggestions. I will take a look at these.
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