How can I accurately determine and standardize the concentration of Macrophomina phaseolina spores or mycelial fragments for inoculation?

I have incubated my fungi for 10 days, then scraped and sieved it using a strainer. I prepared dilutions of 1:10, 1:20, and 1:50, and measured absorbance at 650 nm using a spectrophotometer. I...

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