How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to do Statictical analysis for unequal sample sizes?

Hi Everyone, I have a dataset from proteomics analysis of treated cancer cell line, I have three replicates in treatment time-points and two replicate in control condition. Now, How can I...

01 January 2021 4,055 3 View

Degenerated semiconductor (Burstein Moss effect) with unchanged band-gap?

Is it possible to produce a degenerate semiconductor using a dopant agent that results in increasing the electrical conductivity, but the optical band-gap remains unchanged? In such a situation...

11 December 2020 8,338 13 View

What is the physical concept behind the relationship between the wavelength of light and the particle size in the interaction between them?

Why does light interact with the free electrons of a nanoparticle when its wavelength is larger than the dimensions of the nanoparticle?

13 September 2020 9,044 4 View

The reason for this error in Norsar software???

This error is in the interactive wavefront Tracer section. Tracing process failed: Received job failed indication from initial configuration job: Tracer process (C:/Program Files/NORSAR...

10 July 2020 6,344 3 View

How can I dry aluminum flake without the danger of ignition?

In order to produce Al flake in the ball mill, it is kept under an organic solvent. how can we remove it later from this solvent?

06 July 2020 6,232 7 View

Has anyone ever taken crystal of meta boric acid or B NMR ?

I'm making a boron-containing by-product which in only soluble in DMSO. I've been trying to make a crystal of that. I'm kind of sure is meta boric acid and I have a peak at around 20 ppm in B NMR.

05 February 2020 2,197 2 View

Do you have any suggestions to know about FIELD WORK required for Rock Mass Rating (RMR) and Slope Mass Rating?

For RMR and SMR classifications, field work is required to obtain information about discontinuity dip, strike and ,.... Are are aware of any resource to learn this field work?

14 January 2020 3,307 8 View

Do we use only Octanol for calculating of logP? Do we use methanol or any other organic compounds instead of Octanol?

do we use only Octanol for calculating of logP? Do we use methanol or any other organic compounds instead of Octanol?

20 October 2019 7,022 3 View

Which additive can create tone (especially green) in white ABS masterbatches to omit the undesirable yellow color ?

white ABS masterbatch usually has bluish or greenish color. optical agents lead to green tone in white masterbatch and omit inherent yellow color of ABS but will decompose during the process.

11 October 2019 3,740 1 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

How can I remove bubble in WPU film?

I am trying to prepare water-based polyurethane films. When dried air bubbles were formed and destroys the film homogeneity, then I can't use this for mechanical test because of defects. Is there...

26 January 2021 701 1 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How to model bubble coalescence and/or departure from a surface in ANSYS Fluent?

Dear all, I would like to simulate the pool boiling (subcooled). Specifically, I'm interested in departure of bubbles from finned surfaces. I want to do it in ANSYS-Fluent. Is it possible? if so,...

14 January 2021 6,304 5 View

Best way to get air out of a hamilton syringe?

I'm using a hamilton syringe to inject mineral oil through PVC tubing attached to the needle tip of the syringe. To make sure the tubing stays free of bubbles, I have to get all the air out of the...

10 January 2021 8,082 1 View

Why is PVDF splashing and bursting under vacuum ?

I prepared PVDF in a very regular way by dissolving it with stirring at room temperature but when i placed it in vacuum for eliminating the bubbles and trapped gases it started to burst and...

09 January 2021 3,965 3 View

Bubble column design?

A bubble column of 10 m height and 1m diameter (implying high hydrostatic pressure difference) is being fed leisurely with a flow rate of 10 L/min. What is the resulting dissolved oxygen profile...

31 December 2020 524 1 View

How to model bubble in Ansys Fluent?

I am seeking to model a simple bubble growth/collapse in Ansys. I have tried using 2D, VOF with an initial patch air circle geometry surrounded by water. I include surface tension and make the air...

10 December 2020 9,113 3 View