How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to do Statictical analysis for unequal sample sizes?

Hi Everyone, I have a dataset from proteomics analysis of treated cancer cell line, I have three replicates in treatment time-points and two replicate in control condition. Now, How can I...

01 January 2021 4,055 3 View

The reason for this error in Norsar software???

This error is in the interactive wavefront Tracer section. Tracing process failed: Received job failed indication from initial configuration job: Tracer process (C:/Program Files/NORSAR...

10 July 2020 6,344 3 View

How can I dry aluminum flake without the danger of ignition?

In order to produce Al flake in the ball mill, it is kept under an organic solvent. how can we remove it later from this solvent?

06 July 2020 6,232 7 View

Has anyone ever taken crystal of meta boric acid or B NMR ?

I'm making a boron-containing by-product which in only soluble in DMSO. I've been trying to make a crystal of that. I'm kind of sure is meta boric acid and I have a peak at around 20 ppm in B NMR.

05 February 2020 2,197 2 View

Which additive can create tone (especially green) in white ABS masterbatches to omit the undesirable yellow color ?

white ABS masterbatch usually has bluish or greenish color. optical agents lead to green tone in white masterbatch and omit inherent yellow color of ABS but will decompose during the process.

11 October 2019 3,740 1 View

How to choose the proper neural network Classifier in MATLAB?

I want to use a neural network classifier to separate patients and healthy persons by some parameters. and then test validation of the classifier. Is svm a proper tool? And how can I test the...

21 October 2018 8,968 8 View

Dose anyone know why ht29/5 fu resistant cells don't grow?

17 November 2016 6,149 3 View

How can I solve this RACE PCR problem to make the band visible?

01 January 2015 8,545 4 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What are the available keys and descriptions for the identification of lice and fleas in domesticated animals?

I am an undergraduate student at the University of Peradeniya Sri Lanka, currently working on ecto-parasites in domesticated animals. I found difficulties in the identification due to a lack of a...

15 February 2021 277 1 View

How to improve results of system identification procedure?

Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...

07 February 2021 9,791 2 View

How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

30 January 2021 3,081 3 View

What is the best universal primer for genus bacterial identification?

I would like to ask whether anybody can offer the best universal primer for genus bacterial identification??

23 January 2021 9,017 3 View