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Questions related from John Yn
I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler). However,...
29 May 2024 3,639 0 View
When trying to do RMCE (recombinase-mediated cassette exchange), I add the new DNA sequence with LoxP and Lox2272 sites along with Cre recombinase, so then it switches out genes from my original...
10 April 2024 4,010 0 View
I see it added to some formulations, but when I search around online for what it does I don't see clear answers! I also see conflicting information on whether it's good or bad for endothelial cells.
22 June 2022 8,400 5 View
I was wondering if algae with a lot of oil produced float to the top of their suspension culture based bioreactors - wouldn't this mess up the bioreactor system? (e.g algae all bunched to the top...
12 May 2021 5,439 2 View
Currently we have 4-6 perfusion chambers and we're having to set up individual perfusion loops for each chamber to ensure that the same flow rate is applied to each one - is there a way to...
04 October 2020 6,721 1 View
Hi everyone, I'm currently trying to embed endothelial cells in 3D and have them form capillaries (vasculogenesis). However, the cells often look like they're dead after embedding! Has anyone...
07 September 2020 8,875 10 View
Hi everyone, I've been performing some 3D cultures where I embed cells in hydrogels. When I have many sample groups, I find that the cells that I detach stay in a suspension for quite some time...
18 August 2020 5,051 0 View
Hi everyone! I'm currently using C3H 10T1/2 cells to act as pericytes/support cells for vessels formed in 3D by HUVECs. However, I see that people also use other cells (smooth muscle cells [SMCs],...
05 August 2020 3,579 2 View
Hi everyone, Currently I'm culturing cells in a 3 mg/ml fibrin hydrogel. However, the cells either destroy the gels (without any anti-fibrolytic compounds) or compact the gels when anti-fibrolytic...
02 August 2020 8,271 1 View
I see that there are tests for looking at meat water holding capacity that invovles pressing meat on filter paper, or centrifuging samples. However, how does these tests distinguish between water...
16 January 2020 2,863 3 View
Hi everyone, I saw that 3T3-F442As are supposed to differentiate into adipose better than 3T3-L1s, but I've also read about people having problems with the cells detaching after reaching...
11 December 2019 3,287 0 View
Hi everyone, I'm having trouble getting protein expression with mRNA that I'm transfecting into my cells. I was wondering if mRNA secondary structure could be affecting things, so I inputted my...
26 November 2018 2,101 2 View
Hi everybody, I've tried multiple times to make some fibrin gels, but they always seem to gel on me. What's strange is that part of the solution seems to gel instantly, while the rest gel slower...
21 September 2018 3,494 6 View
Hey everyone, I see my expected ~6 kb band in my gel after linearizing my plasmid (acquired from DH5alpha cells via a plasmid purification kit). However, I also see 4 extra 0.5-1 kb bands. The...
22 August 2018 5,075 2 View
