The basic idea behind Reverse Vaccinology is that an entire pathogenic genome can be screened using bioinformatics approaches to find genes. Next, those genes are filtered for desirable attributes that would make good vaccine targets such as outer membrane proteins. Those proteins then undergo normal wet lab testing for immune responses. I wish to know how extending this can be helpful in producing vaccines for the Swine Flu and Hep-B? Please share your comments and opinions.
You may be best served by getting an advance degree in immunology. Furthermore, there are vaccines for Swine flu and Hepatitis B available. Finding a more general vaccine for influenza is certainly a "holy grail" in immunology and will certainly be aided by Reverse Vaccinology. Attenuation are aided as well..
Dear Richard Cronkhite Sir,
I am very much grateful for your words and I request to kindly refer me any Journal article helpful to me in understanding well about the reverse vaccinology action, mechanism and development.
Here is one: http://www.ncbi.nlm.nih.gov/pubmed/15994562
Science. 2005 Jul 1;309(5731):148-50.
Identification of a universal Group B streptococcus vaccine by multiple genome screen.
Maione D, Margarit I, Rinaudo CD, Masignani V, Mora M, Scarselli M, Tettelin H, Brettoni C, Iacobini ET, Rosini R, D'Agostino N, Miorin L, Buccato S, Mariani M, Galli G, Nogarotto R, Nardi Dei V, Vegni F, Fraser C, Mancuso G, Teti G, Madoff LC, Paoletti LC, Rappuoli R, Kasper DL, Telford JL, Grandi G.
SourceChiron srl, Via Fiorentina 1, 53100 Siena, Italy.
Abstract
Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens.
Hi!!
Also it coul be useful for you this one. I think if you write to this author, he could send you the plasmid!
Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR. "Universal primer set for the full-length amplification of all influenza A viruses." Arch Virol (2001) 146: 2275-2289
Regards,
Briefly, if you know the protein that is immunogenic start studies by bioinformatics, observe hydrophobicity, hydrophilicity, coil, in the same; after the selection is completed using the differents tools use MHC. Subsequently can synthesize and test sequences for B and T epitopes using PBL of animals inoculated with the intact protein under study. Then select the best, designs genes with preferential codons for the vector and you get the subunit. Later test the same in animal target or model. As you can see is delayed but it is a way to use the reverse vacinologia for obtention of subunit vaccines
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