Specifically in terms of pulsing conditions, recovery medium, and incubation time?
I want to design a reconfigurable transmit array using varactor diode with metamaterial unit cell. I have gone through many literatures but in most of literature only different design and...
02 December 2023 1,948 2 View
How to report that in APA format? What has to be done further?
24 April 2023 5,126 7 View
Is there any way?
06 June 2022 8,978 3 View
If we have highly sheared and hydrothermally altered metasediments, what are the criteria using which we can differentiate between detrital, metamorphic and hydrothermally formed allanite grains?
23 December 2021 2,306 4 View
I initiated LCLs 8 weeks ago with 1ml EBV/flask and RPMI with 20%FBS. We are facing the following problems. 1.The cells are small in size ,not expanding as frequently as suggested by established...
02 July 2021 4,368 2 View
Hi, I need some reference of Journals which have monthly publication, in the filed of customer care/ luxury segment /consumer insights /CRM 1) Scopus index 2) UGC approved journal - India...
22 October 2020 7,946 3 View
Hi everyone, does anyone have experience with performing tail vein injection with the breast cancer cell line BT549? I am looking for some advice on what the metastasis burden looks like, what...
14 October 2020 442 2 View
Is it possible to differentiate xenotime from bastnaesite using optical properties?
30 July 2020 1,882 0 View
is there any method to know the age of an ultramafic body which is now thoroughly altered to serpentinite?
22 March 2020 7,347 0 View
the ability of hydrotherms produced during thermal metamorphism to mobilize elements largely depends on its oxygen fugacity. Can anyone guide me what exactly are the factors controlling oxygen...
20 October 2019 7,045 8 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
I'm currently working on gel purification in order to get DNA fragment for T4 ligation. But I have been able to take only 8.5ng/μl as a highest concentration of DNA fragment. And could not get...
01 July 2024 4,912 9 View