What difference between hydrolyzed collagen type I and II ?
and the source is has significant effect?
how can detect a hydrolyzed collagen is which type?
I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...
16 February 2021 5,876 2 View
Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...
21 January 2021 2,593 1 View
Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...
21 December 2020 9,978 3 View
Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...
14 December 2020 1,801 2 View
I am working in research on climate adaptation so need to compare the performance of the urban fabric to the climate changes, which requires me to make a comparison and simulation of the...
03 December 2020 988 8 View
Dears, I have different variables in multiple regression. Two variables are highly correlated. To solve the collinearity issue, I dropped one of them from the multiple regression model. The...
29 November 2020 2,695 4 View
I need this because I have to represent it in a conference as a keynote speaker
17 November 2020 584 4 View
I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...
16 November 2020 9,455 2 View
It is easy to understand the meaning of relationship and effect (impact) in general. But when it comes to the statistical analysis it is a difficult conundrum, as one can determine the...
24 July 2020 6,525 7 View
We are aware of the fact that some type of solvent is required for isolating a compound from herbs. I personally believe that water is the best solvent since mostly decoction is prepared using...
22 July 2020 4,179 2 View
I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...
07 February 2021 5,487 3 View
I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...
24 January 2021 8,009 3 View
Hello all, I am searching for a way to measure the activity of CPT1 and/or FAO in mouse hematopoietic stem cells, which means I have very little material available to do so. Any...
20 January 2021 8,499 2 View
I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...
06 January 2021 8,487 2 View
I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...
01 January 2021 239 1 View
I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...
27 December 2020 5,495 3 View
what is the best method to determine the dominant orientation of collagen fibers? i preformed FFT and shited the frequencies , what should be my next step ?
24 December 2020 3,360 3 View
We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...
21 December 2020 2,454 3 View
I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...
16 December 2020 3,935 2 View
dear all, Actually, I m trying to prepare hydrogel using fish collagen, rat tail collagen, and bovine collagen to check the cell adhesion and viability. fish and rat tail collagen nicely formed as...
15 December 2020 7,142 4 View