Might be higher concentration induce heavy stress which will lead to escaping mutation which inturn more growth and hence adverse effect
Proliferation..it show high yield than control mainly negative control( cell+ media)
Can you please be a bit more specific in which drug and which cell type (line) are you talking about? Without such key information it is nearly impossible to build a viable hypothesis.
This can happen due to the unspecific inhibition of regulatory or feedback loops in signal pathways. For example at a very low does the drug A can specifically hit target X. However, if the target X is inhibited by another isoform Y or Z and if your drug at high concentrations hits all of these enzymes then overall outcome can be relieving all the regulatory pathways resulting in the cell growth or proliferation or perhaps more survival.
I agree with Ezra - you are hitting multiple targets here, but that's about as much as one can tell about the targets with so little input about the nature of the treatment.
actually iam working with plant extract as drug in different cancer cell line like MCF-7 and other.but i seen a pattern of increased growth in higher conc. i assuming that it is interfering some growth factor or cell cycle signalling molecules.
In such a case, you are probably dealing with effects of multiple compounds, at least one of them highly active against one target, while another (or others?) working only at the higher concentration on (possibly) different target and having a dominant effect (pro-proliferative). That was the simplest possibility. In reality, you are probably dealing with many active ingredients, and until you separate this mixture into individual components and test those one by one, I am afraid little else can be added. It's like reading tea leaves, unfortunately. Sorry I can't offer more.
As you know, a crude plant extracts will have many active metabolites. The binding sites might vary. Some might have a dose response relationships. There is a possibility of having pro-proliferative, inhibitory, or even synergistic effects. Additionally, the efficacy of these compounds might vary according to the nature of the soil. For example, the potency of ginseng grown in different part of the world might have different pharmacological properties. I agree with the argument by Ezra and Vadim. You can use concentration gradient to see what happens. You can develop a dose response curve, etc. You can try to see specific and non-specific effects. At different concentration, you could be down regulating something and at higher concentration you could be upregulating some metabolites/factors..meaning a global disinhibition. However, the fact of the matter is, you're using a crude extract. It is difficult to say exactly what is happening. You have to revisit your hypotheses.
Now with Different dose iam checking expression of some signalling molecules..
I think sometimes it can be a false result when you use a mtt test. mtt dye itself can be reduced by some componds such as different antioxidants or other drugs. this induce to a false result. also microbial contamination in drug suspension used for serial dilution in mtt test can induce high absorbtion in higer drug concentrations and low absorbtion in lower dug concentration according to contamination severity.
I have same problem in my antibacterial studies with echinoderm crude extract, if anybody know publications to justify these answers, please provide the details.
I am facing similar pattern of cytotoxicity anyone with literature explaining this phenomenon
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