How are intragenic lncRNAs distinguished from pre-mRNA or debranched introns by qPCR?
lncRNA are spliced, so primers can be designed specifically for them.
I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
17 July 2024 8,330 1 View
I need to get the Technical Data Sheet of these 2 type of alloys .
20 May 2024 4,464 1 View
Can anyone in pharmaceutical development help me with some questions ? 1) When developing in R&D a STERILE topical form (ointment, cream, gel) what should be the requirements for the...
21 March 2024 2,562 4 View
Traditional methods for the determination of agar content in macroalgae need a lot of time, if there is a fast and accurate agarine content detection method will greatly promote the development of...
21 March 2024 3,306 0 View
According to Ahmed (2022), "Urgent revitalization of the centralized national blood transfusion service, as the only permanent solution to the aforementioned battery of challenges, is hereby...
06 September 2023 166 1 View
I would like to find the number of citations for a few particular papers and also list my publications in order of citation numbers. I cannot see how to do that. Is there a users' guide somewhere? .
29 August 2023 8,621 3 View
Hi everyone, it's my first post here and am new to SEM. :) Anyway, I would like some help and recommendations on how to improve the CFA model I'm working on. The CFA is based on n=310 with a...
13 July 2023 5,910 3 View
How to synthesis manganese doped graphene oxide after the synthe of graphene oxide by Hummers method ?
04 June 2023 3,844 3 View
Our group has been having difficulties finding a consistent mechanism by which to screen Bt isolates for Cry or Cyt toxin production in a quantitative, reasonably high-throughput manner. Does...
21 May 2023 276 0 View
I have googled without any luck
29 April 2023 6,749 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View