How are calculations of GSH and MDA concentrations in liver and kidney tissue of laboratory rats ??

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Şükriye Deniz Mutluay

The level of malondialdehyde can be measured in the liver, kidney, brain, and plasma by using the thiobarbituric acid method and by derivatizing with 2.4 dinitrophenylhydrazine using the HPLC method.

Mohammad Sheibani

Hi,

Tissue levels of GSH and MDA are measurable with ELISA method. After separating the supernatant of these tissues. This method is valuable using ELISA reader.

Good luck