Hello! My name is Laura. I have been working on the generation of colon organoids from patient biopsies for the last two years. I follow standard protocols, first I isolate intestinal crypts and culture them in matrigel. We know the matrigel cultures do not contain stromal components such as fibroblast and immune populations. Now we would like to generate organoids with it´s native infiltrated immune component, and for that we plan to stablish an air liquid interface (ALI) culture, which according with published literature, they preserve all the stromal populations. Anybody can explain the biological reason for that? What´s the difference between matrigel and ALI cultures in that sense?

Thanks in advance!

Laura.