Does anyone know the best way to get a homogenate of mouse brain?
Can I do it just by using cold PBS and a few freeze-thaw cycles?
I used this method many years ago, it is reliable and quick. I suggest starting with spinal cord but you can dissect out brain white matter if you wish.
J Neurochem. 1986 May;46(5):1644-6.
A rapid method for purification of myelin basic protein.
I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...
22 February 2021 1,400 4 View
I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....
19 February 2021 8,414 4 View
Hello everyone. How can I simulate evaporative cooling between air and water (isothermal) placed in direct contact? Is it possible to choose or set the humidity difference as the heat exchange...
11 November 2020 5,225 3 View
Hello everyone I need to simulate a counter-current flow between air and water droplets (in direct contact) which by evaporative cooling give rise to a cooling of the wall on which the droplets...
07 November 2020 5,458 2 View
Hi, I am doing a research with Theory of Planned Behavior. The behavior is attending a course/training on the subject of orientation, navigation and topography in the next year. I completed...
18 August 2020 4,502 1 View
Greetings Everyone. I am reading several articles about biochars and notice several use N2 atmosphere inside the muffle furnace. can you produce biochars without this N2 atmosphere or other inert...
17 August 2020 8,992 6 View
Dear all, I need to process several classified GIS maps in FRAGSTATS. All files are in GeoTiff format. I do not have ArcGIS. I also assume that when you export files to ASCII format in QGIS often...
01 July 2020 2,433 5 View
Dear all, I need to prepare electrical contacts in order to connect a gold pad with a semiconductor (few microm size) . i cannot perform lithographic process. Hence, how can I obtain good...
09 June 2020 9,756 8 View
Do you know if (anonymised) clinical high-frequency datasets of COVID-19 patients, such as pulmonary function, blood oxygen level, blood pressure, cardiac interbeat interval etc., are available...
04 May 2020 2,195 5 View
Cheers Everyone. I was seeing some uses of ionic exchange to recover NH4 by ionic exchange with other cations in a specific matrix. And it brought me to question what drives an ionic exchange to...
17 February 2020 6,596 3 View
03 March 2021 8,272 1 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View