Homemade buffer for 4D- NucleofectorTM System (Lonza)?

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Where to get .cif for MoS2 bilayer in AA- and AB-stacking?

Hello researchers , I am working with 2D materials. I need .cif of Bilayer MoS2 in AA-3R and AB-2H stacking. Please recommend a source. Thank you.

06 November 2023 9,385 1 View

Photosynthesis is exothermic or endothermic process?

I am wondering if photosynthesis is an exothermic or endothermic process. What I knew that it is an endothermic process because photosynthesis needs energy in the form of radiation to prepare...

31 October 2023 8,984 2 View

C2000 TI F2806x PIL simulation is working extremely slow, any possible solution?

I am simulating a feedback control strategy for power converters, the model is simulated in discrete time with a sampling rate of 1e-6. The simulation for 1 sec is taking only 2 secs in standalone...

13 June 2023 937 0 View

How to calculate built-in electric potential in Janus 2D materials using quantum espresso?

Hi everyone, I am doing DFT study on Janus 2D materials using quantum espresso. These materials exhibit built-in electric field perpendicular to their surface which tune their properties. How this...

07 April 2023 9,604 0 View

How to resolve the contact problem of ballistic impact LS-DYNA?

Hi, I am working on a ballistic impact study on a hybrid kevlar metal armor optimization using data from published literature. Currently, I'm building a baseline model consisting of only kevlar...

04 April 2023 1,087 0 View

Can not access file with extension ".a2r". NEED RECOMMENDATION PLZZ?

Hello I have got my samples analyzed by FTIR. The result file bears the extension .a2r and I am unable to open it. Even I searched on google but could not resolve the issue. Any body can help in...

29 March 2023 5,518 2 View

How to solve "c_bands: 4 eigenvalues not converged" problem in quantum espresso SCF calculations?

Hello researchers, I am doing DFT via Quantum espresso 7.1, usually during convergence tests or actual calculations I usually encounter a problem in SCF.out stating that "c_bands: __(1,2,3 or...

24 March 2023 7,854 2 View

What are the new research trends / hot topics in IoT security?

Hello Folks! IoT security is becoming a key area of research these days. However, to move into a certain direction of research is hard to decide before knowing its value in the future. What do...

15 January 2023 7,250 8 View

Does anyone have a nurses' performance questionnaire ?

I am currently working on my research " The relationship between nurse leadership style and Staff Nurses' Performance " and I need a questionnaire to assess performance of nurses.

24 August 2022 7,465 5 View

2. PhD Dissertation Sruvey?

Dear Respondent, I am inviting you to participate in research conducted for academic purposes to investigate the relationship between animosity and consumer perceptions. Included with this...

17 December 2021 6,103 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View