Homemade buffer for 4D- NucleofectorTM System (Lonza)?

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Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View

Do you think that COVID-19 is completely disappear after vaccine discovery?

one year age and COVID-19 was the main subject that distracts the human minds. before few days, a vaccine was completing its trial experiment to be used in prevention among human beings. do you...

07 December 2020 9,422 8 View

Do you agree with beall s list of predatory journals?

some said that beall s list is not an official web-page, so not all reported information are true. do you agree or disagree with this? and how you evaluate its list of journals and publishers?

12 August 2020 9,543 5 View

Journals interested in publishing about coronavirus disease?

do you have any idea about the name of journals interested in publishing articles related to COVID-19?

22 May 2020 4,842 8 View

What are the specific enzymes which plays pivotal role in iso-peptide bond formation like Transglutaminases?

Enzymes from the lyase, ligase, and hydrolase classes have the ability to catalyze carbon-carbon bond formation. Likewise, I want to know about specific enzymes (like Transglutaminases) or class...

20 February 2020 5,862 3 View

Hi.. what is the best wireless network protocol for video transmission ?

I need to know the new the network techniques that provide fast video or image transmission

20 February 2020 4,694 6 View

Reformation and Improvement of Higher Education System: Academic Community?

Please friends suggest your opinions on how could you reform the higher education systems (Universities)... Thank you very much and I appreciate it for you all. Best Wishes Aqeel

29 November 2019 7,683 4 View

I have one question regarding Lickert-Scale Questionnaire analysis?

I have one question regarding Lickert-Scale Questionnaire analysis in Patient satisfaction. the main outcome was observed as either patients satisfied or not satisfied so my question is, as...

12 September 2017 6,160 5 View

What is the best method of vascular anastomosis in recipients with a tortuous external iliac artery?

As the artery lies medial to the vein, there are 2 problems 1 where to site the venous anastomosis, and 2 the arterial anastomosis I cant find any references on the subject?

08 July 2017 9,021 5 View

Is it possible to isolate exosomes from live cells (not fluids) and how could i do that?

Protocol

08 March 2016 5,782 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View