HL-1 cells are famous for being the only contracting cell-line. They proliferate while keeping a differentiated cardiomyocyte phenotype. However, concerns about their genetic stability is considerable. Keeping them beating passage after passage is very tricky (any tips on that?). The increasing population of non-beating cells in the culture have different metabolic profiles and have basically lost the only advantage of these cells.
On the other hand, H9c2 cells are much cheaper and easier to maintain. But they are undifferentiated myoblasts, so they do not contract and are generally considered less relevant to cardiomyocytes.
Any ideas about which one is preferred over the other?
Depends on what your research focus is. Remember the hl1 cells are derived from the mouse atria. while although derived from myoblasts, h9c2 cells are supposed to be of left ventricle origin and rat.
Also keep in mind the hl1 cells have adenosine in the media, a known cardio protective agent.
I feel both could be used as good supporting studies to cardiomyocytes but are difficult to rationalize their validity as stand alone cell lines to support a hypothesis without other techniques.
Totally agree Eric. Both has limitations that must be considered.
The adenosine point is interesting though. What else does this media include. Is the formula published anywhere?
HL-1 cells need to reach full confluence, and only then can be split 1:3. Also what most people and I consider to be 100% confluence is not enough, and one has to change medium and let it go another day. You will see cells tightly pressed against each other and more than 1 cell spontaneously beating per 10x microscopy view. I was told that this regimen of passaging helps to minimize time the cells spent disconnected from each other. Check with Claycomb lab and they'll say the same.
My opinion is that HL-1 has an advantage over adult or neonatal isolated when you use lentivirus to stably transduce them to be GFP or luciferase reporter lines, then puromycin or something else to select out non-transduced or low expressors. That, or if your experimental treatments are long (24h++) such as with chemotherapeutic agents, where isolated adult cells would die off even in vehicle controls.
I am in the process of also trying to figure out which to use H9c2 vs HL-1. I will be transiently transfecting them. Does anyone have any suggestion on the best protocol to use for that?
See this post for HL-1 transfection...
https://www.researchgate.net/post/Does_anyone_know_how_to_improve_HL-1_transfection_efficiency_using_lipofectamine_and_weather_these_cells_behave_the_same_way_without_coating
Does anyone know if the H9c2 will contract in response to electrical stimulation?
Does anyone have a protocol to induce beating in H9C2 differentiated with RA?
induction of H9C2 cell beating .... is it possible with retinoic acid induced differention?
Dear fellows,
I am looking for HL-1 cells. Dr. Claycomb just replied me an email saying they cannot send them out at this time.
Does anyone know any lab I could get them from?
thanks in advance.
Murilo
Which ones are better for studying ion channels? What is your experience? What about traffick of ion channels in HL-1 vs. H9C2?
Carmen, I studied VGCC and SOCE in HL-1 cells. I know they have been patch clamped as well. Not sure about the H9C2....
Iam studying the effect of lamin A mutation associated with Dilated cardiomyopathy on nuclear morphology. Which cell line HL-1 or H9C2 will be a better model to study?
Has anyone tried to transfect HL-1 cells with lentiviral constructs?
Hi I am Youping from university of California Sanfrencisco. I have got the HL-1 cell from Dr. William C. Claycomb, Ph.D. in 2009. But by some reason, we lost the cells duce to a lab move. I have tried to contact to Dr. Claycomb again, but all the emails have rejected or undeliverable. I have called his office, there is no luck too. Is anyone has the HL-1 cells and could sure some aliquot to me. Your help will be highly appreciated. Pleas contact me at [email protected]. I will send you the FedEx account number for shipping and the shipping address.
Thanks millions
Youping He, PhD
University of California, San Francisco
School: UCSF School of Medicine
Department: Pediatrics, Pediatric Critical Care
Campus Address Box 1346
513 Parnassus Ave HSE 1401 San Francisco, CA 94143
Phone (415) 476-8392
Email: [email protected]
depending on your experimental settng you habve to choose amurine cell line (HL-1, most often used, standard) or rat cells (H9C2).
I would propose to use HL-1 cells, because it is a often veryfied and used standard model.
- Badges
- Science topic