Greetings
Does anyone know the history of E. coli XL10-gold (strategene)? Did it originate from XL1-Blue and what was the procedure? If you have a reference even better.
Yoram
E. coli are gram-negative, rod shaped bacteria that were named after Dr. Theodor Escherich, the scientist who first described them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally occurring strains of E. coli, some of which are deadly to humans. The majority of all common, commercial lab strains of E. coli used today are descended from two individual isolates, the K-12 strain and the B strain. K-12 was isolated from a patient in 1920 and eventually led to the common lab strains MG1655, which led to DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred to as “B strain” in 1942. The BL21 strain, (and derivatives) are the most common examples of the E. coli B strain.
See the link; you may find desired information -
http://blog.addgene.org/plasmids-101-common-lab-e-coli-strains
According to the Agilent's manual, XL2-blue is a derivative of XL1-blue, and XL10-Gold is a derivative of XL2-Blue MRF ́.
https://www.agilent.com/cs/library/brochures/5989-8281ENUS.pdf
"The XL10-Gold® ultracompetent cells were designed to transform large plasmids and ligated DNA with the highest transformation efficiency possible, while exhibiting faster growth and larger colonies. This strain was created by moving the Hte phenotype into our highest-efficiency strain, XL2-Blue MRF ́. "
Through, I've noticed a notable decrease of firefly luciferase expression from pLR vectors in XL10-gold compared to XL2/XL1, so it may have more extensive differences.
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